BLUE-COLORED GOLD NANOPARTICLE FOR IMMUNOLOGICAL MEASUREMENT, METHOD FOR PRODUCING THE SAME, AND MEASUREMENT METHOD USING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide: a gold nanoparticle including an organic buffering agent having a piperazine ring, gold, and an organic acid having reducing properties, while a solution of the gold nanoparticle dispersed as a colloidal solution exhibits a blue color in visual observation; a method for producing the gold nanoparticle; and an immunological measurement method using the gold nanoparticle as a labeling particle.

SOLUTION: A nuclei formation step of reacting an organic buffering agent having a piperazine ring with a solution of a first gold salt to form a nuclear gold nanoparticle and then a growth step of growing the nuclear gold nanoparticle by simultaneously adding a solution of a second gold salt and an organic acid having reducing properties to a solution of the nuclear gold nanoparticle to cause a reaction are performed. Thus, a gold nanoparticle can be easily produced, while a solution in which the gold nanoparticle is dispersed as a colloidal solution exhibits a blue color in visual observation.

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