| 摘要 |
This instant invention is based on the discovery that pluripotent human stem cells maintained under the defined culture conditions can be uniformly converted into a specific lineage by small molecule induction. Retinoic acid induces the specification of neuroectoderm direct from the pluripotent state of human stem cells and triggers progression to neuronal progenitors and neurons efficiently. Similarly, nicotinamide induces the specification of cardiomesoderm direct from the pluripotent state of human stem cells and triggers progression to cardiac precursors and cardiomyocytes efficiently. This technology provides a large supply of clinically-suitable human neuronal or cardiac therapeutic products for CNS or myocardium repair. This invention enables well-controlled efficient induction of pluripotent human stem cells exclusively to a specific clinically-relevant lineage for tissue and organ engineering and regeneration, cell-based therapy, and drug discovery. |
