METHOD FOR DISCRIMINATING BETWEEN CYNANCHUM WILFORDII AND CYNANCHUM AURICULATUM AND A KIT THEREFOR
摘要
<p>PURPOSE: A method for distinguishing Cynanchum wilfordii and Cynanchum auriculatum is provided to enable distinguishing Cynanchum wilfordii and Cynanchum auriculatum by direct sequencing, SNP genotyping, and RFLP. CONSTITUTION: A method for distinguishing Cynanchum wilfordii and Cynanchum auriculatum using RFLP analysis comprises a step of preparing a biological sample from Cynanchum wilfordii and Cynanchum auriculatum; a step of isolating genome DNA from the biological sample; a step of amplifying DNS sequence having a sequence of sequence number 1 and amplifying DNA sequence of sequence number 2 of Cynanchum auriculatum; and a step of treating the amplified products with Xcml and detecting the cleaved DNA fragment.</p>
申请公布号
KR20120062614(A)
申请公布日期
2012.06.14
申请号
KR20110109104
申请日期
2011.10.25
申请人
JEJU TECHNOPARK
发明人
JUNG, YONG HWAN;OH, DAE JU;LEE, CHANG HOON;PARK, JEE KWON;BU, HEE JUNG;KIM, DAE SHIN;PARK, SOO YEONG;LEE, WOOK JAE;KIM, BONG CHAN;LEE, SANG SOON;OH, HAN JUN