| 摘要 |
PURPOSE: A method for isolating beta-sitosterol from Hippophae rhamnoides L. stems is provided to obtain a therapeutic agent for treating various inflammatory diseases including arthritis. CONSTITUTION: A method for isoalting beta-sitosterol from Hippophae rhamnoides L. stems comprises: a step of dipping dry Hippophae rhamnoides L. stems in ethanol and applying ultrasonic wave; a step of collecting supernatant to prepare ethanol extract; a step of adding distilled water and hexane to the ethanol extract and collecting supernatant to prepare a hexane fraction; a step of adding dichloromethane to prepare a dichloromethane fraction; a step of adding ethyl acetate to upper layer isolated from the solution to prepare an ethyl acetate fraction; a step of adding butanol to a lower layer isolated from the solution containing ethyl acetate to prepare a butanol fraction; a step of preparing a distilled water fraction from the solution; a step of mixing the hexane fraction, dichloromethane fraction, ethyl acetate fraction, butanol fraction, and distilled water fraction to prepare a mixture fraction; and a step of performing middle pressure liquid chromatography of the mixture fraction using a first solution containing hexane, dichloromethane, ethyl acetate and butanol and a second solution containing methanol.
|
