发明名称 |
System and method for providing enhanced background rejection in thick tissue with differential-aberration two-photon microscopy |
摘要 |
A system for providing enhanced background rejection in thick tissue contains an aberrating element for introducing controllable extraneous spatial aberrations in an excitation beam path; at least one mirror capable of directing received laser pulses to the aberrating element; an objective; a beam scanner imaged onto a back aperture of the objective so that the beam scanner steers beam focus within the thick tissue; and a detector for recording signals produced by the tissue. An associated method comprises the steps of acquiring two-photon excited fluorescence of thick tissue without extraneous aberrations; introducing an extraneous aberration pattern in an excitation beam path; acquiring two-photon excited fluorescence of the thick tissue having the introduced extraneous aberration pattern; and subtracting the two-photon excited fluorescence with extraneous aberrations from the acquired standard two-photon excited fluorescence of the thick tissue without extraneous aberrations. |
申请公布号 |
US8198604(B2) |
申请公布日期 |
2012.06.12 |
申请号 |
US20080240074 |
申请日期 |
2008.09.29 |
申请人 |
MERTZ JEROME;TRUSTEES OF BOSTON UNIVERSITY |
发明人 |
MERTZ JEROME |
分类号 |
G01N21/64 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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