PREPARATION METHOD OF ANTIGEN-IMMOBILIZED IMMUNOFLUORESCENCE SLIDE, AND IMMUNOFLUORESCENCE SLIDE PREPARED THEREBY

摘要

The present invention relates to a preparation method of an antigen-immobilized immunofluorescence slide comprising the following steps: immobilizing a C-reactive protein on a slide to prepare a protein chip; mixing an antibody, which specifically binds to a protein to be analyzed, and streptavidin to label the same with fluorescent nanoparticles; carrying out the immune reaction of the antibody by competitive mixing; and carrying out analysis by using a fluorescent camera, and an immunofluorescence slide prepared thereby, wherein the step of immobilizing a C-reactive protein on a slide comprises the following steps: preparing a slide modified with 3-aminopropyltrimethoxysilane; hydrating the slide modified with 3-aminopropyltrimethoxysilane; activating the modified slide by using a glutaraldehyde solution; dissolving a C-reactive protein in 30-70 mM phosphate buffer solution (pH 6.5-7.8) by a concentration of 0.01-0.5 mg/mL to prepare an antigen solution for immobilization; putting a Petri dish containing the slide on a spotting guide and spotting 1-100 µL of the prepared antigen solution on a dropping point; and reacting the slide prepared by the step for 1-6 hours to immobilize the antigen.