发明名称 |
COVALENTLY DIMERIZED BIVALENT BINDING AGENTS |
摘要 |
<p>The present invention addresses limitations of prior art receptor-based traps through a methodology called the clamp/click/cleave (CCC) approach. Two fusion proteins each comprising a binding domain fused to a coiled-coil are non-covalently dimerized through the coiled-coil (clamp), and the dimer so formed is stabilized by a covalent disulphide bond (click) between cysteine residues located on the fusion proteins between the binding domains and coiled-coils. Once the disulphide bond has formed, the coiled-coils are subsequently removed (cleave) by cleaving the fusions proteins at cleavage sites located between the cysteine residues and the coiled-coils to provide the covalently dimerized bivalent binding agent of the present invention. Such binding agents are useful in the treatment and diagnosis of disease states characterized by production and/or overexpression of a ligand to which the binding domains bind. The invention is particularly useful for covalently dimerized receptor-based ligand traps where the binding domains are receptor ligand-binding domains, such as those of TGF-ß receptors.</p> |
申请公布号 |
CA2819187(A1) |
申请公布日期 |
2012.06.07 |
申请号 |
CA20112819187 |
申请日期 |
2011.11.28 |
申请人 |
NATIONAL RESEARCH COUNCIL OF CANADA |
发明人 |
ZWAAGSTRA, JOHN C.;O'CONNOR-MCCOURT, MAUREEN D.;SULEA, TRAIAN;COLLINS, CATHERINE;BANVILLE, MYRIAM;JARAMILLO, MARIA |
分类号 |
C07K19/00;A61K39/395;A61K47/48;A61P35/00;C07K14/705;C07K16/00;C07K16/46;C12P21/00;G01N33/574 |
主分类号 |
C07K19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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