发明名称 |
MICROBIAL PRODUCTION OF POLYHYDROXYALKANOATES |
摘要 |
<p>A process for producing medium-chain-length 3-hydroxyalkanoic acids in a liquid medium, the process comprising: (i) intermixing a liquid culture medium and a microbial inoculum comprising a pure culture of a Pseudomonas putida strain having a portion of a phaC1 gene deleted and/or a portion of a phaC2 gene deleted and/or a portion of a ?ha? gene deleted, (ii) culturing the mixture for a period of time to allow for microbial cell growth to occur in the liquid culture medium thereby producing a spent liquid medium and a plurality of microbial cells wherein medium-chain-length 3-hydroxyalkanoic acids are synthesized, (iii) separating the medium-chain-length 3-hydroxyalkanoic acids from the plurality of microbial cells and the spent liquid medium; and (iv) recovering the separated medium-chain-length 3- hydroxyalkanoic acids. A pure culture comprising transgenic microbial cells wherein a portion of a phaC1 gene and/or a phaC2 gene and/or a phaZ gene has been deleted.</p> |
申请公布号 |
WO2012071657(A1) |
申请公布日期 |
2012.06.07 |
申请号 |
WO2011CA01322 |
申请日期 |
2011.11.29 |
申请人 |
UNIVERSITY OF MANITOBA;LAVIN, DAVID B.;SHARMA, PARVEEN |
发明人 |
LAVIN, DAVID B.;SHARMA, PARVEEN |
分类号 |
C12N1/21;C12N1/20;C12N15/00;C12P7/42 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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