HOT-START PCR BASED ON THE PROTEIN TRNS-SPLICING OF NANOARCHAEUM EQUITANS DNA POLYMERASE

摘要

PURPOSE: A hot-start PCR method based on protein trans-splicing of Neq L fragment and Neq S fragment is provided to be used in a technique of preventing carryover contamination. CONSTITUTION: A hot-start PCR method based on protein trans-splicing of NeqL fragment and NeqS fragment containing intein comprises: a step of preparing a PCR reaction solution; a step of adding the NeqL fragment and NeqS fragment; a step of performing trans-splicing to form a polypeptide with NeqDNA polymerase activity; and a step of performing DNA denaturation, primer annealing, and elongation.