METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS IN TRANSGENIC ANIMALS

摘要

A method is disclosed of increasing the biological activity of a Vitamin K dependent protein produced in a transgenic animal, cell or cell line comprising feeding a transgenic animal or cell /cell line expressing a gene encoding the Vitamin K dependent protein an animal feed or media comprising an increased amount of vitamin K over that of standard animal feed containing a standard amount of vitamin K. The Vitamin K dependent protein expressed in the transgenic animal possesses an increased biological activity over a Vitamin K dependent protein expressed in a transgenic animal that is fed standard animal feed or standard media containing a standard amount of Vitamin K where the biological activity is increased as a result of an increase of gamma carboxylation of the Vitamin K dependent protein.