DETERMINATION OF THE RISK OF DISTANT METASTASES IN SURGICALLY TREATED PATIENTS WITH NON-SMALL CELL LUNG CANCER IN STAGE I-IIIA

摘要

A method of determination of the risk of distant metastases in surgically treated patients with non-small cell lung cancer in stage I-IIIA based on that a sample of primary tumor tissue is acquired, from which at least one microRNA is extracted which retrotranscripted into complementary DNA (cDNA) by reverse transcription, wherefore microRNA in examined sample is quantificated with the use of quantitative PCR method while the expression value of each microRNA is referred to the reference expression values in a recurrence prediction model in which the expression values are correlated to the high and low risk of distant metastases is characterized by that the expression value of only one from twenty two microRNAs listed below in Table 1 in primary tumor tissue of non-small cell lung cancer is measured.