Method of seprating and purifying nicotineamide adenine dinucleotide phosphate-cytochrome P450 and Kit thereof

摘要

PURPOSE: A method for selectively concentrating, isolating, and purifying NADPH-cytochrome P450 reductase is provided to develop a novel drug. CONSTITUTION: A method for isolating and purifying NADPH-cytochrome P450 reductase comprises: a step of crushing cells and centrifuging; a step of removing supernatant and collecting precipitate containing membrane proteins; a step of isolating the membrane proteins with a complex detergent of CHAPS(3-[(3-cholamido propyl)dimethyl ammonio]-1-propan sulfate) and polyglycol ether surfactant(Tergitol NP-10); and a step of selectively isolating the membrane protein using Ni2+-nitrilotriacetic acid(NTA) agarose column.