发明名称 |
Method for delivering nucleic acid into eukaryotic genomes |
摘要 |
The present invention relates to genetic engineering and especially to the use of DNA transposition complex of bacteriophage Mu. In particular, the invention provides a gene transfer system for eukaryotic cells, wherein in vitro assembled Mu transposition complexes are introduced into a target cell and subsequently transposition into a cellular nucleic acid occurs. The invention further provides a kit for producing insertional mutations into the genomes of eukaryotic cells. The kit can be used, e.g., to generate insertional mutant libraries. |
申请公布号 |
US8192934(B2) |
申请公布日期 |
2012.06.05 |
申请号 |
US201113229258 |
申请日期 |
2011.09.09 |
申请人 |
SAVILAHTI HARRI;FRILANDER MIKKO;MENG XIAOJUAN;PAATERO ANJA;PAJUNEN MARIA;TURAKAINEN HIKKA;FINNZYMES OY |
发明人 |
SAVILAHTI HARRI;FRILANDER MIKKO;MENG XIAOJUAN;PAATERO ANJA;PAJUNEN MARIA;TURAKAINEN HIKKA |
分类号 |
C12Q1/68;C12N9/22;C12N15/10;C12N15/90 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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