| 发明名称 |
Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase |
| 摘要 |
The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5′ end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5′ end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA. |
| 申请公布号 |
US8192932(B2) |
申请公布日期 |
2012.06.05 |
| 申请号 |
US20100658764 |
申请日期 |
2010.02.12 |
| 申请人 |
SHUMAN STEWART;SEKIGUCHI JOANN;COMISKEY JOHN;FERNANDEZ JOSEPH;HOEFFLER JAMES;MARCIL ROBERT;SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH;INVITROGEN CORPORATION |
发明人 |
SHUMAN STEWART;SEKIGUCHI JOANN;COMISKEY JOHN;FERNANDEZ JOSEPH;HOEFFLER JAMES;MARCIL ROBERT |
| 分类号 |
C12N15/09;C12Q1/68;C07H21/02;C07H21/04;C12N9/90;C12N15/10;C12P19/34;C12P21/06 |
主分类号 |
C12N15/09 |
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