QUANTITATIVE ANALYSIS METHOD USING A NANOTUBE HAVING INTEGRATED ENZYMES

摘要

The present invention relates to a quantitative analysis method which involves analyzing an object using an enzyme, aptamer, antibody, nanotube, and a magnetic bead or a substrate. More particularly, a binding aptamer or antibody to be bonded to an analysis object is bonded to a magnetic bead or a substrate, and the resultant structure is placed into a solution to be analyzed, bonded to the analysis object, and separated from the analysis object by magnetic force; another aptamer or antibody to be selectively bonded to the analysis object is bonded to the surface of the nanotube, on which enzymes are integrated, so as to prepare for a quantitative analysis of the analysis object; and the quantitative analysis is performed, through an enzyme reaction, on a composite body which is prepared by binding the resultant structure to the magnetic bead or substrate to which the aptamer or antibody is bonded. Thus, a low concentration analysis object can be diagnosed, and difficulties in quantitative analysis according to conventional detection methods can be overcome using the signal-amplification effects of a nanotube having integrated enzymes.