METHOD AND APPARATUS FOR FRAGMENTING NUCLEIC ACIDS
摘要
Systems and methods include processing devices used to fragment nucleic acids to average nucleic acid sizes ranging from between about 5 kbp and about 20 kbp. A processing device may include an inlet portion and a channel upstream from a shearing region arranged so that a relatively constant pressure is established and maintained (e.g., a pressure that changes by less than about 40%) at an entrance of the shearing region during a majority of sample flow through the shearing region. In some embodiments, after forcing the sample through the shearing region once, the processing device may be taken out of the centrifuge, inverted and placed back into the centrifuge so that the sample is forced through the shearing region again.
申请公布号
WO2012040571(A3)
申请公布日期
2012.05.31
申请号
WO2011US52959
申请日期
2011.09.23
申请人
COVARIS, INC.;DURIN, GUILLAUME;LAUGHARN, JAMES, A., JR.