Methods for producing hyaluronan in a recombinant host cell

摘要

METHODS FOR PRODUCING HYALURONAN IN A RECOMBINANT HOST CELL Abstract The present invention relates to methods for producing a hyaluronic acid, comprising: s (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an m isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences 5 encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP N-acetylglucosamine pyrophosphorylase. (I 141768_1):KZA