发明名称 METHOD FOR SENSITIVE DETECTION OF EML4-ALK FUSED GENE
摘要 <P>PROBLEM TO BE SOLVED: To provide a method for a highly sensitive and accurate detection of EML4-ALK fusion gene that may serve as a lung cancer marker in a very small amount of a sample that may have damage on a specimen like a paraffin embedded specimen (nucleic acid, and particularly, mRNA), and to provide a primer and kit for use in the method. <P>SOLUTION: The detection method includes: (A) a step of preparing a template DNA from a sample; and (B) a step of carrying out amplification within an amplifiable base sequence region by using a combination of amplification primers for amplification in a region including a fusion point with respect to the template DNA. <P>COPYRIGHT: (C)2012,JPO&INPIT
申请公布号 JP2012100628(A) 申请公布日期 2012.05.31
申请号 JP20100254179 申请日期 2010.11.12
申请人 MITSUBISHI CHEMICAL MEDIENCE CORP;KINKI UNIV 发明人 NISHIO KAZUTO;NAKAGAWA KAZUHIKO;OKAMOTO ISAMU;ARAO TOKUZO;SAKAI KAZUKO;SEKIJIMA MASARU
分类号 C12Q1/68;C12N15/09 主分类号 C12Q1/68
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