摘要 |
In a STED fluorescence light microscope pulses of excitation light (3) are applied to a sample, which excite fluorescent entities contained in the sample for fluorescence, and which are focused on at least one focal area. Further, de-excitation light (12) is applied to the sample, which de-excites the excited fluorescent entities and which comprises an intensity zero point in the at least one focal area, as a continuous wave. Fluorescence light emitted by the excited fluorescent entities in the sample is registered after each pulse of the excitation light (3) and overlapping with applying the de-excitation light (13) with high temporal resolution between consecutive pulses of the excitation light (3). |
申请人 |
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.;HELL, STEFAN W.;ENGELHARDT, JOHANN;REUSS, MATTHIAS;WESTPHAL, VOLKER;EGGELING, CHRISTIAN;MONERON, GAEL;HAN, KYU-YOUNG;VICIDOMINI, GIUSEPPE |
发明人 |
HELL, STEFAN W.;ENGELHARDT, JOHANN;REUSS, MATTHIAS;WESTPHAL, VOLKER;EGGELING, CHRISTIAN;MONERON, GAEL;HAN, KYU-YOUNG;VICIDOMINI, GIUSEPPE |