发明名称 GENETIC TESTING METHOD FOR CANCER BY ANALYSIS OF EXPRESSION OF CANCER-RELATING GENE UTILIZING MONOCYTE CONTAINED IN BLOOD SAMPLE
摘要 <P>PROBLEM TO BE SOLVED: To provide a genetic testing method capable of accurately predicting a risk of occurrence of a cancer cell mass or the effect of a therapy in an early stage or after prognosis. <P>SOLUTION: The genetic testing method for cancer includes: a step 10 for separating a buffy coat layer from a blood sample by centrifugation; a step 20 for separating a monocyte by density gradient centrifugation; a step 30 for extracting total RNA from the monocyte; a step 40 for synthesizing complementary DNA from the total RNA by reverse transcription reaction; a step 50 for carrying out a real-time PCR(RI-PCR) analysis for a cancer-relating gene; and a step 60 for carrying out a DNA microarray analysis for the cancer-relating gene by employing a DNA microarray as a nucleic acid microarray. <P>COPYRIGHT: (C)2012,JPO&INPIT
申请公布号 JP2012100536(A) 申请公布日期 2012.05.31
申请号 JP20090048342 申请日期 2009.03.02
申请人 GENESCIENCE CO LTD 发明人 FUJISAKI KOJI;KAWASAKI HIROAKI
分类号 C12Q1/68 主分类号 C12Q1/68
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