发明名称 |
GENE TARGETING KNOCK-IN VECTOR CONTAINING OVEREXPRESSION CASSETTE, THE METHOD FOR CONSTRUCTING THE SAME AND TRANSGENIC CLONED ANIMAL CARRYING THE VECTOR FOR XENOTRANSPLANTATION |
摘要 |
PURPOSE: A gene targeting knock-in vector including protein over-revelation cassette, a manufacturing method thereof, and a transformation cloned animal for heterograft in which the vector is introduced are provided to manufacture a transformation reproduction miniature reproduction pig in which a graft rejection is controlled by effectively knock-out and knock-in at the same time. CONSTITUTION: A gene targeting knock-in vector successively including protein over-revelation cassette comprises a first area having base sequences which are homologous in gene of mammal, a cassette for protein over-revelation, and a second area having homologous base sequences in gene of mamma. The gene of mammal is alpha 1,3- galactosyltransferase gene. The mammal is a pig. The pig is a NH-miniature pig. The first area is a left arm including a part of intron 8 of the alpha 1,3- galactosyltransferase or Exxon 9. A size of the first area is 2kb~6kb.
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申请公布号 |
KR20120055409(A) |
申请公布日期 |
2012.05.31 |
申请号 |
KR20100117142 |
申请日期 |
2010.11.23 |
申请人 |
REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION) |
发明人 |
OH, KEON BONG;HWANG, SEONG SOO;IM, GI SUN;KWAK, TAE UK;WOO, JAE SEOK;YANG, BYOUNG CHUL;KO, YEOUNG GYU;KIM, KYUNG WOON;PARK, SOO BONG;KANG, MAN JONG |
分类号 |
C12N15/85;A01K67/027;C12N15/54 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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