EXPRESSION, PURIFICATION AND REFOLDING OF RECOMBINANT CHLAMYDIA PROTEINS, COMPOSITIONS AND RELATED METHODS

摘要

The present invention provides methods of obtaining a recombinant Chlamydia outer membrane protein (e.g. MOMP) that is expressed as an insoluble aggregated in a heterologous host in a soluble and immunogenic form and to proteins prepared in accordance to these methods. The invention also provides compositions comprising one or more proteins prepared in accordance to the methods of the invention. The proteins obtained in accordance to the methods of the present invention are soluble, immunogenic and in a substantially oligomeric form.