发明名称 |
In Vitro Production Of Oligodendrocytes From Human Umbilical Cord Stem Cells |
摘要 |
The invention provides a method of producing oligodendrocytes by in vitro differentiation of human multi-potent progenitor cells (MLPCs). The method comprises culturing isolated MLPCs on a first surface in a serum-free defined culture medium; replacing the culture medium with serum-free culture medium supplemented with bFGF, EGF and PDGF-AA for approximately 24 hours; changing the cultured MLPCs into the supplemented serum-free culture medium further supplemented with differentiation factors norepinephrine, forskolin. and K252a; establishing a 3D environment by covering the culture with a second surface opposite and spaced apart from the first surface, so as to contain the MLPCs therebetween; and continuing to culture until a majority of the MLPCs have differentiated into oligodendrocytes. Additionally included is a method of treatment for a subject afflicted by a disease characterized by central or peripheral nervous system demyelination, the method comprising transplanting into the subject oligodendrocytes produced according to the method disclosed. |
申请公布号 |
US2012128639(A1) |
申请公布日期 |
2012.05.24 |
申请号 |
US201013322903 |
申请日期 |
2010.05.28 |
申请人 |
HICKMAN JAMES J.;DAVIS HEDVIKA;UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC. |
发明人 |
HICKMAN JAMES J.;DAVIS HEDVIKA |
分类号 |
A61K35/30;A61P25/00;C12N5/079 |
主分类号 |
A61K35/30 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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