FORMULA FOR TNFR-IG FUSION PROTEIN

摘要

PROBLEM TO BE SOLVED: To provide an improved system for large scale production of proteins and/or polypeptides in cell culture.

SOLUTION: An improved system for large scale production of proteins and/or polypeptides in cell culture is especially characterized by one or more of: (i) a cumulative amino acid concentration greater than about 70 mM per unit volume; (ii) a molar cumulative glutamine to a cumulative asparagine ratio of less than about 2; (iii) a molar cumulative glutamine to a cumulative total amino acid ratio of less than about 0.2; (iv) a molar cumulative inorganic ion to a cumulative total amino acid ratio between about 0.4 to 1; and (v) a combined cumulative glutamine and cumulative asparagine concentration greater than about 16 mM per unit volume.

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