发明名称 |
Methods and Devices for Rapid Detection and Identification of Live Microorganisms by Aptamers and/or Antibodies Immobilized on Permeable Membranes |
摘要 |
The invention provides methods, devices and kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing nutrient media. The invention includes mixing one or more target microorganisms with one or more aptamers and/or one or more antibodies, each conjugated to a reporter compound and specific for a first site on one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and/or one or more antibodies, each specific for a second site on one or more target microorganisms or a site on the aptamer conjugate and/or antibody conjugate. A detection solution is added to the membrane and detection and identification of one or more target microorganisms is achieved in about one hour or less.
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申请公布号 |
US2012129157(A1) |
申请公布日期 |
2012.05.24 |
申请号 |
US201213350127 |
申请日期 |
2012.01.13 |
申请人 |
BARNHIZER BRET T.;GAZENKO SERGEY |
发明人 |
BARNHIZER BRET T.;GAZENKO SERGEY |
分类号 |
C12Q1/70;C12M1/34;C12Q1/68;G01N33/569;G01N33/577 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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