发明名称 |
DETECTION OF BACTERIAL (MOLLICUTES) CONTAMINATION |
摘要 |
The present disclosure provides a method and system for the PCR amplification of a target sequence which suppresses non-specific amplification products. The disclosure concerns the use of a primer pair optimized to amplify a nucleic acid of a contaminant in the background of genomic DNA of a first organism. When DNA from a second organism suspected for comprising the contaminant is subjected to the same PCR-based amplification reaction, detection sensitivity and specificity of the contaminant is enhanced when an amount of genomic DNA of the first organism is present in the amplification reaction.
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申请公布号 |
US2012129181(A1) |
申请公布日期 |
2012.05.24 |
申请号 |
US201213364050 |
申请日期 |
2012.02.01 |
申请人 |
BIRKNER CHRISTIAN;ROCHE DIAGNOSTICS OPERATIONS, INC. |
发明人 |
BIRKNER CHRISTIAN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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