| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. <P>SOLUTION: The method includes a step of identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods are employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The humanized antibody is used in a variety of therapeutic, diagnostic, and research applications. <P>COPYRIGHT: (C)2012,JPO&INPIT |
