| 摘要 |
In a first aspect, the present invention provides a nucleic acid expression construct encoding a fusion protein comprising a fluorescence reporter protein and a protein with a wild-type destruction signal, wherein the sequence encoding the fusion protein is operably linked to a non-endogenous promoter, and wherein the fusion protein localizes during cell cycle progression to subcellular structures selected from the group consisting of the cell cortex, the contractile ring, and the midbody. In a further aspect, the present invention provides a method of visualizing cell cycle progression, said method comprising the steps of (a) introducing the nucleic acid expression construct of the present invention into a cell; (b) expressing the fusion protein encoded by the nucleic acid expression construct within the cell; (c) and monitoring the expression of the fusion protein by means of fluorescence analysis. In another aspect, the present invention provides the use of the nucleic acid expression construct of the present invention as a cell proliferation marker in vivo and in vitro. In yet another aspect, the present invention provides a transgenic animal comprising the nucleic acid expression of the present invention as well as its use for analyzing cell proliferation in vivo. |
