Synthesis method of aromatic amino acids

摘要

A method for synthesizing aromatic amino acids according to one aspect of the present invention includes processes of: (a) of preparing a thermostable a Thermus thermophilus aspartate aminotransferase by culturing an E. coli BL21(DE3) cell transformed with a vector comprising a gene encoding the Thermus thermophilus aspartate aminotransferase; (b) contacting the thermostable Thermus thermophilus aspartate aminotransferase of (a) with an amino donor and an amino acceptor at a temperature range of 50-80° C. to obtain an aromatic amino acid; (c) precipitating the aromatic amino acid of (b); and (d) recovering the thermostable Thermus thermophilus aspartate aminotransferase.