METHOD FOR ENZYMATIC HYDROLYSIS OF ORGANOPHOSPHOROUS SOIL COMPOUNDS

摘要

FIELD: medicine. ^ SUBSTANCE: what is offered is a method for enzymatic hydrolysis of organophosphorous soil compounds. The method involves introducing a biocatalyst - raw polyhistidine - containing a polypeptide characterised by the organophosphate hydrolase properties, prepared of the bacterial strain Escherichia coli CKMIBH 29 and immobilised on a cellulose containing carrier. The biocatalyst is introduced in the concentration of 0.5-1.0 g/kg at pH 6.5-10.0 and temperature 15-40C in the soil containing organophosphorous compounds in the concentration up to 850 mg/kg. ^ EFFECT: method provides complete decomposition of wide spectrum of the OPCs at high specific hydrolysis rate. ^ 1 dwg, 8 ex