SYSTEMS AND METHODS FOR IMMUNOSORBENT ASSAYS FOR SINGLE AND MULTIPLE ANALYTES

摘要

Systems and methods for minimizing or eliminating steps in immunosorbent assays for single and multiple analytes by eliminating the need to attach target molecules to the test well and to remove unbound antibodies through rinsing. The immunosorbent assay (ISA) is utilized for a single analyte or target and includes the step of mixing immunologic molecules with the sample and detection. Disclosed is an immunosorbent assay for multiple analytes (ISAMA) for testing a plurality of analytes or targets in a single well using a modified ISA test wherein different tags are attached to different antibody pairs. Alternate embodiments use multiple types of scavenger antigens with corresponding elimination of the need for scavenger antibodies. Disclosed are various test wells for rapid simultaneous testing of fluids for a plurality of components and a methodology for a continuous immunosorbent assay. Also disclosed is a method for immunosorbent assay of micro-organisms to determine serotype.