摘要 |
The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker. |
申请人 |
CODEXIS, INC.;CABIROL, FABIEN, L.;GOHEL, ANUPAM;COLLIER, STEVEN, J.;LIANG, JACK;MOCK, MARISSA;MUNDORFF, EMILY;NOVICK, SCOTT;LIMANTO, JOHN;SMITH, DEREK;BEUTNER, GREGORY;KLAPARS, ARTIS;ASHLEY, ERIC;STROTMAN, HALLENA;TRUPPO, MATTHEW;HUGHES, GREGORY;GRAU, BRENDAN;JANEY, JACOB |
发明人 |
CABIROL, FABIEN, L.;GOHEL, ANUPAM;COLLIER, STEVEN, J.;LIANG, JACK;MOCK, MARISSA;MUNDORFF, EMILY;NOVICK, SCOTT;LIMANTO, JOHN;SMITH, DEREK;BEUTNER, GREGORY;KLAPARS, ARTIS;ASHLEY, ERIC;STROTMAN, HALLENA;TRUPPO, MATTHEW;HUGHES, GREGORY;GRAU, BRENDAN;JANEY, JACOB |