| 摘要 |
B. anthracis detection primers can be used to amplify the conserved regions of bclB alleles encoding the collagen-like proteins found in B. anthracis , as opposed to other Bacillus species, during PCR amplification of extracted Bacillus DNA or Bacillus spores. Additionally, B. anthracis strain fingerprinting primers amplify bclA-E polymorphic regions of collagen-like proteins found in B. anthracis strains. The B. anthracis strains differ in basepair size of these polymorphic coding regions of bclA-E so that strains can be discriminated based upon distinct PCR-band patterns that migrate differently in size while resolved on an agarose gel. DRAWINGS |
