发明名称 |
GAUSSIA LUCIFERASE VARIANT FOR HIGH-THROUGHPUT SCREENING |
摘要 |
Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described. |
申请公布号 |
US2012122182(A1) |
申请公布日期 |
2012.05.17 |
申请号 |
US201013381788 |
申请日期 |
2010.06.30 |
申请人 |
TANNOUS BAKHOS A.;MAGUIRE CASEY;THE GENERAL HOSPITAL CORPORATION |
发明人 |
TANNOUS BAKHOS A.;MAGUIRE CASEY |
分类号 |
C12N9/02;C12N1/19;C12N1/21;C12N5/04;C12N5/07;C12N5/071;C12N15/53 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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