| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases. <P>SOLUTION: There are provided a circularly permuted anthozoan luciferase protein and a circularly permuted decapod crustacean luciferase protein, containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further, a modified anthozoan luciferase protein and a modified decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest are provided. <P>COPYRIGHT: (C)2012,JPO&INPIT |
