发明名称 |
METHOD AND APPARATUS FOR MAINTENANCE AND EXPANSION OF HEMOPOIETIC STEM CELLS AND/OR PROGENITOR CELLS |
摘要 |
A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells ; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.
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申请公布号 |
US2012122220(A1) |
申请公布日期 |
2012.05.17 |
申请号 |
US201213360068 |
申请日期 |
2012.01.27 |
申请人 |
MERCHAV SHOSHANA;MERETSKI SHAI;ZIPORI DOV;KADOURI AVINOAM;PLURISTEM LTD |
发明人 |
MERCHAV SHOSHANA;MERETSKI SHAI;ZIPORI DOV;KADOURI AVINOAM |
分类号 |
C12M3/00;C12N5/0789;A61F2/00;A61K35/12;A61K35/14;A61P7/00;A61P43/00;C12M3/04;C12N5/00;C12N5/02;C12N5/06;C12N5/071;C12N5/078;C12N5/08;C12N11/08;C12R1/91 |
主分类号 |
C12M3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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