发明名称 |
DETECTION AND VISUALIZATION OF THE CELL CYCLE IN LIVING CELLS |
摘要 |
<p>The present invention relates to a nucleic acid molecule encoding a polypeptide specifically binding to proliferating cell nuclear antigen (PCNA), said nucleic acid molecule comprising a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence deviating from SEQ ID NO: 2 by conservative substitution of one or more amino acids in position 1 to 28, 38 to 52, 63 to 98 and 115 to 123 of SEQ ID NO: 2. The present invention also relates to a vector comprising the nucleic acid molecule of the invention, a host cell comprising the nucleic acid molecule of or the vector of the invention and a method of detecting the amount and/or location of PCNA in living cells, a method of screening for compounds having an effect on the cell cycle.</p> |
申请公布号 |
EP2451841(A1) |
申请公布日期 |
2012.05.16 |
申请号 |
EP20100730775 |
申请日期 |
2010.07.06 |
申请人 |
LUDWIG-MAXIMILIANS-UNIVERSITAET |
发明人 |
ROTHBAUER, ULRICH;LEONHARDT, HEINRICH;ZOLGHADR, KOUROSH;ROMER, TINA;SCHMIDTHALS, KATRIN |
分类号 |
C07K16/18;C07K14/415;C12N15/13;C12N15/62;C12N15/63;G01N33/53 |
主分类号 |
C07K16/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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