GFP-SPECIFIC SINGLE DOMAIN ANTIBODY

摘要

PURPOSE: A GFP-specific single domain antibody is provided to improve thermal stability by specifically binding to GFP. CONSTITUTION: A GFP(green fluorescent protein)-specific single domain antibody has one kind of amino acid sequence selected from sequence numbers 12 to 23. An amino acid of sequence number 12 is encoded by a base of sequence number 24. An amino acid of sequence number 13 is encodes by a base of sequence number 25. An antibody-conjugated GFP has improved thermal stability. An immunosensor contains GFP. A method for improving thermal stability of GFP comprises a step of maintaining GFP fluorescence expression at 65°C-75°C.