VERFAHREN UND ZUSAMMENSETZUNGEN ZUR HERSTELLUNG VON RNA AUS EINER FIXIERTEN PROBE

摘要

The present invention provides improved methods and compositions for RNA isolation. In particular embodiments the present invention concerns the use of methods and compositions for the isolation of full-length RNA from fixed tissue samples. The present invention provides methods for digesting and extracting RNA from a fixed tissue sample. The methods are based on a digestion buffer comprising a polyanion (e.g. citrate) and a protease (e.g. proteinase K).