发明名称 |
Mutant SepRS, and method for site-specific introduction of phosphoserine into protein using the same |
摘要 |
A mutant SepRS which is suitable for a site-specific introduction of phosphoserine into a protein is prepared by analyzing the structure and functions of a phosphoseryl-tRNA synthetase (SepRS) derived from an archaebacterium. A mutant SepRS composed of an amino acid sequence depicted in SEQ ID NO:2, in which any one or more of glutamic acids at position-418 and position-420 and threonine at position-423 are substituted with other amino acid, and having enhanced binding affinity with a suppressor tRNA as compared with a wild type phosphoseryl-tRNA synthetase (SepRS) composed of an amino acid sequence depicted in SEQ ID NO:2 is provided. |
申请公布号 |
US8178301(B2) |
申请公布日期 |
2012.05.15 |
申请号 |
US20080318344 |
申请日期 |
2008.12.24 |
申请人 |
YOKOYAMA SHIGEYUKI;FUKUNAGA RYUYA;SEKINE SHUN-ICHI;RIKEN |
发明人 |
YOKOYAMA SHIGEYUKI;FUKUNAGA RYUYA;SEKINE SHUN-ICHI |
分类号 |
C12Q1/68;C12N1/21;C12N9/00;C12N15/63 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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