FLUORESCENCE MICROSCOPE FOR MULTI-FLUORESCENCE IMAGE OBSERVATION, FLUORESCENCE IMAGE OBSERVATION METHOD USING THE SAME, AND MULTI-FLUORESCENCE IMAGE OBSERVATION SYSTEM

摘要

Provided are a fluorescence microscope for multi-fluorescence image observation, a fluorescence image observation method using the same, and a multi-fluorescence image observation system. The fluorescence microscope for multi-fluorescence image observation includes: a first light module including a first light source for supplying first excitation light having a first wavelength, a first excitation filter for selectively transmitting the first excitation light supplied from the first light source, a first dichroic filter for reflecting the first excitation light passing through the first excitation filter toward an object to be observed, an objective lens for collecting the first excitation light reflected by the first dichroic filter to transmit the collected excitation light toward the object to be observed, a second dichroic filter for reflecting first emissive light emitted from the object to be observed, a first emission filter for selectively transmitting the first emissive light emitted from the second dichroic filter, and a first-image acquirement part for acquiring an image through a first emissive light supplied through the first emission filter; and a second light module including a second light source for supplying second excitation light having a second wavelength, a second excitation filter for selectively transmitting the second excitation light supplied from the second light source, an objective lens for emitting light passing through the second excitation filter and radiated onto the object to be observed, a second emission filter for selectively transmitting second emissive light passing through the first dichroic filter and the second dichroic filter, and a second image acquirement part for acquiring an image through the second emissive light supplied through the second emission filter. According to the present invention, the fluorescence microscope for multi-fluorescence image observation may include a plurality of image acquirement parts corresponding to the number of light sources for emitting light to easily and quickly observe a plurality of fluorescence images without mechanical movement.