摘要 |
51-Nucleotides are prepared by contacting an enzyme source, having ribose-51-phosphate transferring activity for producing 51-nucleotide from the corresponding organic base and a 51-nucleotide, with a solution containing the corresponding organic base and a 51-nucleotide other than that to be synthesized, and allowing biochemical reaction to take place to produce the desired 51-nucleotide. Suitable microorganisms which can be used are enzyme sources which belong to the genera Pseudomonas, Alcaligenes, Serratia, Flavobacterium, Micrococcus, Staphylococcus, Proteus, Aerobacter, and Escherichia. The organic bases which may be used are the purine and pyrimidine bases such as purine, adenine, hypoxanthine, xanthine, guanine, 2-aminopurine, 2,6-diaminopurine, 6-dimethyl aminopurine, benzyladenine, 6-methylaminopurine, kinetin, uracil, 5-hydroxy-methyluracil, cytosine, thymine and barbituric acid. The enzyme reaction may be carried out in aqueous medium of a pH of from 5 to 10 and a temperature of from 30 DEG to 60 DEG C. and the synthesized 51-nucleotide isolated from the reaction mixture by such methods as ion exchange chromatography or precipitation. 51-nucleotide may suitably be obtained as crude crystals in the form of the alkali metal salt. The enzyme source can be admixed with the reaction mixture in the form of cultured broth, living cells, dried cells or cell extracts. |