ACETYLCHOLINESTERASE INHIBITORS FROM ACORN OF QUERCUS ACUTISSIMA

摘要

PURPOSE: A method for preparing acetylcholine esterase inhibitor from arcon is provided to ensure simply obtain a large amount of gallic acid derivatives. CONSTITUTION: A method for preparing acetylcholine esterase inhibitor comprises: a step of pulverizing and freeze-drying acorn; a step of mixing 1 weight part of dry acorn and 80-120 weight parts of mixture solvent of spirit and water; a step of refluxing at 70-90°C for 3-5 hours; a step of mixing 1 weight part of the extract and 10-50 weight parts of distilled water to prepare mixture suspension; a step of adding 80-120 weight parts of first organic solvent and shaking for 20-60 minutes to remove water; a step of dissolving the concentrate in second organic solvent and removing insoluble material; and a step of extracting the materials of chemical formulas 1-3 using LH 20 column chromatography. The extract contains a material of chemical formulas 1-3.