发明名称 |
ENHANCED PRODUCTION OF PAPILLOMAVIRUS-LIKE PARTICLES WITH A MODIFIED BACULOVIRUS EXPRESSION SYSTEM |
摘要 |
The present invention is concerned with the provision of a method for manufacturing papillomavirus like particles (PV-VLP), comprising the steps of a) culturing a host cell lacking protease activity and comprising an expression vector, wherein said expression vector comprises at least one polynucleotide encoding a PV L1 polypeptide, and b) obtaining VLPs from the host cell. Also proposed is a host cell lacking protease activity and comprising an expression vector, wherein said expression vector comprises a polynucleotide encoding at least one PV L1 polypeptide. Furthermore, a method for the manufacture of a pharmaceutical composition for the treatment or prevention of PV-related disease comprising the steps of manufacturing PV-VLPs and the further step of formulating the VLPs as a pharmaceutical composition is proposed as well as an expression vector comprising at least one polynucleotide encoding a PV L1 polypeptide but lacking a functional gene for a v-cath protease.
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申请公布号 |
US2012115207(A1) |
申请公布日期 |
2012.05.10 |
申请号 |
US201013257248 |
申请日期 |
2010.03.30 |
申请人 |
SENGER TILO;MULLER MARTIN;GISSMANN LUTZ;DEUTSCHES KREBSFORSCHUNGSZENTRUM |
发明人 |
SENGER TILO;MULLER MARTIN;GISSMANN LUTZ |
分类号 |
C12N7/02;C12N5/10;C12N15/85 |
主分类号 |
C12N7/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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