PHAGE PHI 29 DNA POLYMERASE CHIMERA

摘要

The present invention is encompassed within the biotechnology field. Specifically, it relates to a DNA polymerase chimera comprising an amino-terminal (N-terminal) region encoding a Æ29 type DNA polymerase and a carboxyl-terminal (C-terminal) region comprising at least one HhH domain which are bound by means of a connecting amino acid sequence and to the use thereof for replicating, amplifying or sequencing a template DNA. Likewise, the present invention provides a method for replicating, amplifying or sequencing a deoxyribonucleic acid with said DNA polymerase chimera and a kit for carrying out said method.