| 摘要 |
The present invention provides a method for detecting and analyzing cells having gene abnormality in a highly accurate and simple manner using FISH. The present invention relates to a method for analyzing genetically abnormal cells, the method including the steps of (a) preparing a cell sample containing eukaryotic cells, (b) conducting an antigen-antibody reaction to cells contained in the cell sample using one or more antibodies which specifically bind to a molecule existing on the cell surface of eukaryotic cells after the step (a), (c) subjecting the cells contained in the cell sample to a permeation treatment after the step (b), (d) subjecting the cells contained in the cell sample to an immobilization treatment after the step (b), (e) conducting FISH to the cells contained in the cell sample using one or more nucleic acid probes after the step (d), (f) analyzing fluorescence signals from the nucleic acid probes in the cells contained in the cell sample using a three-dimensional image analysis method after the step (e), and (g) determining whether the cells contained in the cell sample are genetically abnormal cells or not based on the results of the step (b) and the results of the step (f). |
