In situ dilution of external controls for use in microarrays

摘要

An array in which an external control feature for normalization has been designed and tested for its ability to mimic the range of observed expression levels for a test set of oligos. The external control probes span a series of concentrations. They are spatially randomized across a grid of an array. The series of concentrations is duplicated in a given grid. The individual grid layout and number of control and external normalization features per grid have been designed to cope with sources of both systematic error and spatial variation.