发明名称 |
Method of determining metabolism of a test agent |
摘要 |
A method for detecting one or more metabolites of a test agent in a tissue including: A) incubating a test agent and an enzyme within a three-dimensional structure comprising a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, and wherein the first mold or polymer scaffold has microchannels that form a fluidic branching network that mimics the forces and transport of natural vasculatures and wherein the second mold or polymer scaffold comprises cells; B) forming an enzyme-substrate complex between the enzyme and the test agent; and C) detecting one or more metabolites of the test agent. |
申请公布号 |
US8173361(B2) |
申请公布日期 |
2012.05.08 |
申请号 |
US20100707469 |
申请日期 |
2010.02.17 |
申请人 |
VACANTI JOSEPH P.;RUBIN ROBERT;CHEUNG WING;BORENSTEIN JEFFREY T.;THE GENERAL HOSPITAL CORPORATION;THE CHARLES STARK DRAPER LABORATORY |
发明人 |
VACANTI JOSEPH P.;RUBIN ROBERT;CHEUNG WING;BORENSTEIN JEFFREY T. |
分类号 |
C12Q1/00;A01N1/00;C12N5/00;C12Q;C12Q1/26;C12Q1/34;C12Q1/42;C12Q1/44;C12Q1/48;G01N33/50;G01N33/53 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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