发明名称 |
METHOD FOR OBTAINING HUMAN MICROGLIAL PRECURSOR CELLS FROM PLURIPOTENT STEM CELLS |
摘要 |
A method for obtaining human microglial precursor cells, comprising: (a) providing a cell population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors; (c) expanding and enriching microglial precursor cells in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors and 10 to 150 ng/ml GM-CSF; and (d) isolating microglial precursor cells comprising CD45-positive cells. |
申请公布号 |
US2012107898(A1) |
申请公布日期 |
2012.05.03 |
申请号 |
US201013266845 |
申请日期 |
2010.04.28 |
申请人 |
NEUMANN HARALD;ROY KRISTIN;BRUESTLE OLIVER;PEITZ MICHAEL |
发明人 |
NEUMANN HARALD;ROY KRISTIN;BRUESTLE OLIVER;PEITZ MICHAEL |
分类号 |
C12N13/00;C12N5/071 |
主分类号 |
C12N13/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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