METHOD FOR SETTING TEMPERATURE OF POLYMERASE CHAIN REACTION AND INSTRUMENT THEREOF

摘要

A method for setting temperature of polymerase chain reaction is provided, comprising placing a tube with mixed liquor and DNA sample in an airtight shell, and heating the mixed liquor located in the bottom of the tube to denaturation temperature by using a heater. Thermal convection may take place in mixed liquor due to temperature gradient, and make the temperature of mixed liquor alter continuously during the convection process, thus polymerase chain reaction may take place circularly along with the convection of mixed liquor. Using the method of the present invention, time of polymerase chain reaction can be shortened, and cost of temperature setting instrument is low.